Journal: Journal of Orthopaedic Translation

Article Title: Multi-scale biomimetic fusion construction of cerium ion hydrogel-scaffold for promoting osteoporotic bone defect repair

doi: 10.1016/j.jot.2025.08.015

Figure Lengend Snippet: Evaluation of bone regeneration efficacy in osteoporotic femoral models following 4-week therapeutic intervention. (A) Schematic representation of experimental model establishment and tissue collection protocol. (B) Three-dimensional micro-CT reconstruction analyses were conducted to evaluate neovascularization in the distal femur region (n = 4). (C – E) Histomorphometric quantification parameters including bone volume fraction (BV/TV), intertrabecular distance (Tb.Sp), and trabecular structural thickness (Tb.Th) were systematically measured in distal femoral specimens (n = 4). (F) Histopathological examination using H&E staining visualized tissue morphology (n = 6). (G&I) Modified trichrome staining (MTS) demonstrated collagen deposition patterns in bone repair areas (n = 5). (H – J) Immunohistochemical detection of COL I and RUNX2 expression (Scale bar: 100 μm). Statistical significance thresholds: ∗ p < 0.05, ∗∗ p < 0.01 (two-tailed ANOVA with Tukey post hoc).

Article Snippet: All specimens underwent high-resolution micro-CT scanning (SkyScan 1272, Bruker MicroCT, Belgium) with species-specific parameterization: Murine scans achieved 9 μm isotropic voxel resolution, whereas rat specimens were imaged at 18 μm spatial resolution, maintaining standardized acquisition parameters (50 kv X-ray potential, 200 μA beam current, 0.7° rotational increment through 180° angular range).Volumetric reconstruction was conducted through NRecon v1.7.4.2 software (Bruker), followed by 3D morphological rendering via Mimics Innovation Suite v10.01 (Materialise NV, Leuven).

Techniques: Micro-CT, Staining, Modification, Immunohistochemical staining, Expressing, Two Tailed Test

Journal: Journal of Orthopaedic Translation

Article Title: Multi-scale biomimetic fusion construction of cerium ion hydrogel-scaffold for promoting osteoporotic bone defect repair

doi: 10.1016/j.jot.2025.08.015

Figure Lengend Snippet: The in vivo bone regeneration of osteoporotic femoral bone defects in rats was assessed at 4 and 8 weeks of treatment. (A) Schematic illustration of the bone defect model establishment and tissue sampling protocol. (B) Micro-CT-based quantitative assessment of osteogenesis, including 3D-reconstructed morphometric analysis of neobone formation in femoral defects. (C) Histomorphometric quantification parameters including Bone Mineral Density (BMD), Trabecular Number (Tb.N), and bone volume fraction (BV/TV) were systematically measured in distal femoral specimens (n = 3). Statistical significance thresholds: ∗ p < 0.05 or ∗∗ p < 0.01 (two-tailed ANOVA with Tukey post hoc).

Article Snippet: All specimens underwent high-resolution micro-CT scanning (SkyScan 1272, Bruker MicroCT, Belgium) with species-specific parameterization: Murine scans achieved 9 μm isotropic voxel resolution, whereas rat specimens were imaged at 18 μm spatial resolution, maintaining standardized acquisition parameters (50 kv X-ray potential, 200 μA beam current, 0.7° rotational increment through 180° angular range).Volumetric reconstruction was conducted through NRecon v1.7.4.2 software (Bruker), followed by 3D morphological rendering via Mimics Innovation Suite v10.01 (Materialise NV, Leuven).

Techniques: In Vivo, Sampling, Micro-CT, Two Tailed Test